Agdia, Inc. has added another test kit to their Tomato brown rugose fruit virus (ToBRFV) diagnostic suite. AmplifyRP® XRT for ToBRFV is the second of three ToBRFV diagnostic assays to be launched by Agdia in the first half of 2021. Their high-specificity ELISA assay for ToBRFV was released on January 7. An ImmunoStrip® lateral flow device for ToBRFV is expected to be released in the coming months (Q1 or Q2 of 2021.)
Agdia’s AmplifyRP XRT for ToBRFV has been validated for use with tomato and pepper seeds and leaf. As a rapid, field-deployable molecular method requiring far less training than traditional PCR methods, this assay provides users with greater flexibility to deploy detection capabilities where they need it, when they need it. Use cases for this assay include firstly laboratory-based molecular diagnosis with crude or purified extracts with faster time-to-result than traditional PCR or qPCR methods. This assay can be used with Agdia’s AmpliFire isothermal fluorometer or with most commonly-used real-time PCR machines.
Secondly, the new kit offers in-field monitoring at remote production sites as a stand-alone assay. Directly test ELISA extracts for molecular confirmation of serological screening results, allowing you to act immediately to address production issues.
Agdia’s AmplifyRP XRT for ToBRFV is highly specific to ToBRFV and has been experimentally proven to detect isolates from around the world. No cross-reactivity was observed with high titer samples from other Tobamoviruses, including Cucumber green mottle mosaic virus (CGMMV), Kyuri green mottle mosaic virus (KGMMV), Pepper mild mottle virus (PMMoV), Tobacco mosaic virus (TMV), Tomato mosaic virus (ToMV), Tobacco mild green mosaic virus (TMGMV), Zucchini Green Mottle Mosaic Virus (ZGMMV) and more.
टमाटर ब्राउन रुगोज फ्रुट भाइरस एक प्रतिरोध तोड्ने टोबामोभाइरस हो जसले सोलानम लाइकोपर्सिकम (टमाटर) र क्याप्सिकम एसपीपी लगायत सोलानेसियस बालीहरूमा गम्भीर आर्थिक नोक्सान पुर्याउँछ। (मिर्च)। यसले टोबामोभाइरसका लक्षणहरू निम्त्याउँछ जसमा पातहरूमा मोज़ेक र क्लोरोसिस र फलको रङ्ग र विकृति समावेश हुन्छ। यी लक्षणहरूले उपज घटाउँछ र फल बेच्न नसक्ने बनाउँछ।
टमाटर र काली मिर्चको बीउ, प्रत्यारोपण र निश्चित देशहरूबाट फलफूलहरू संयुक्त राज्यमा USDA-APHIS संघीय आयात आदेशको अधीनमा छन्। टमाटर ब्राउन रुगोज फ्रुट भाइरसलाई EPPO (युरोपियन र मेडिटेरेनियन प्लान्ट प्रोटेक्शन अर्गनाइजेसन) द्वारा क्वारेन्टाइन रोगजनकको रूपमा वर्गीकृत गरिएको छ।
थप जानकारीको लागि:
Agdia Inc।
52642 काउंटी रोड 1
एल्खर्ट, 46514 XNUMX१। मा
www.agdia.com